FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_ibh-30656_rr.352000000a9ad3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_ibh-30656_rr.352000000a9ad3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3200544
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTCGTTA42800.133727266364718No Hit
ATTCATTAGAGTGTGACCCAGCCGTCATAGGAACAGCTGTCAAGGGAAAG39840.12447883859743843No Hit
CTAATGAATAGTCCTCTCTGACCTTCAGCCAAACACTGGTGTGGAAGACC34980.1092939200335943No Hit
TATTTACACAGTGACAAGAAATGCCGGTCTGGTTAAGAGACGTGGAGGTG33080.1033574292370297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTT4800.093.703784
TCGAGTT4800.093.703783
TTCGAGT4850.091.2422
GTTCGAG5400.081.950121
AGTTGCC2650.068.37862145
GTATTTT554.0698596E-665.89212145
TATAGCC1250.063.783577145
GCGACAG1350.059.111561
TGGCGGG2250.054.763676145
TCTATCC5450.050.5809488
CGACAGT1600.049.8745962
ACAGTTC1650.048.3632434
ACCGTCC1602.0008883E-1145.300835145
ACCTCTT2250.045.0995145
ATCTTTT2300.044.119072145
TCTACCC1002.7408896E-643.4888145
AAGCGCC700.001058329441.417908145
TACGTTT700.001058329441.417908145
CGCGGCC1755.2750693E-1141.417904145
AACGCCC1105.277261E-639.53527145
Produced by FastQC (version 0.11.2)