FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_hrv16_rr.352000000a9b75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_hrv16_rr.352000000a9b75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911787
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10620.11647457136370666No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA400.006033184554.4148864
CCCTAAG800.002030297436.2726021
CATCTAG800.002030297436.2726021
CCTTACC4650.028.055803145
GACCCCC2603.7307473E-927.875956145
ACGTGTT5050.025.833559145
CTATACT5250.023.4934124
AGGGCCC3152.842171E-823.0327575
GAGAGCC2903.2622484E-722.5165069
ATGTAAG1800.004291265720.1514471
GTACTAA3301.1297761E-619.7872316
GGGCCCT3301.1297761E-619.7872316
GTATTAG1850.005028376419.6068121
TACTAGG2608.037707E-519.5314032
CTTAGGA2256.838276E-419.3453882
AATGTAC4603.8944563E-918.9269183
CAGGGCC3852.3661232E-718.8449844
TCTCGGG7550.018.239368145
ACTAGGC5209.058567E-1018.1382963
TATAATA2400.001055186418.1363032
Produced by FastQC (version 0.11.2)