FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_hrv14_rr.352000000a9b68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_hrv14_rr.352000000a9b68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951977
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCC1854.1964086E-935.262722145
TCCTACT3150.034.5421942
CTCCTAC3400.029.866811
TATAGGG12100.025.778623
GTATATG30800.023.7853971
TAGGAGA2202.220966E-523.081254
GGGAGTG14100.022.6373127
GTATATA26950.022.0696181
TCTCGGG7250.019.995718145
ATATGAG13700.019.5913835
ATATATC20750.018.5279332
TATATCC32200.018.2475973
CATATAC29100.018.1972793
CTAGGAC9600.018.134963
CGAGGGG3602.6255602E-618.121122145
TATATAC27650.018.1021653
ATATGAC26250.017.9622463
TAGGGAG21000.017.6171155
CTAGCAC9550.017.4703253
TATATGA29550.017.1834262
Produced by FastQC (version 0.11.2)