FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_hb-2016-1840_rr.352000000a9b18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_hb-2016-1840_rr.352000000a9b18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457409
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA67260.2737029122950229No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC55850.22727189491045247No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC45800.186375161806602No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT37190.15133825911763163No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT37060.15080924664962161No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC30830.12545734145191134No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA29890.1216321743755313No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC29840.12142870804168131No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG29730.1209810821072113No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC28740.11695244869698125No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC28120.11442946615724123No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC27380.11141816441626118No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC27350.1112960846159512No Hit
ATATCAGACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAGCCTA26260.10686051853802114No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCC3850.084.71508145
TTCGAGT2350.077.175482
TGACGGG1250.075.3776145
TCGAGTT2450.074.026983
ATTTCGC406.674936E-572.478455145
CGAGTTT2500.069.6459964
GTTCGAG2650.068.437241
ACCTATT902.0560037E-856.37214145
GGCTTCT8600.053.1423151
TCTTCCG9250.049.411015
CGCTCGG450.00965211648.318974145
TAGACGG752.5561647E-548.31897145
CTTCCGT10050.046.199676
CCGAGGG657.349425E-444.602127145
TTCCGTG10500.043.526097
AGGCGCC855.3531505E-542.634388145
GCCTGCC2350.040.094467145
GTCGTAA750.001485975738.655174145
GAAGCGA4200.038.0012866
AAGCGAA4250.037.5519147
Produced by FastQC (version 0.11.2)