FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_fss-13025_rr.352000000a9ac6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_fss-13025_rr.352000000a9ac6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687641
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAA63040.23455513589798635No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC58080.21610029018012453No Hit
GTATATAACACTTATTCATCCCCATTGTGGTTGGAAAAGATATGGCCTCC43430.16159152208200425No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG38030.1414995529536869No Hit
CCCCTGTACCGCATCTCGTTTCCTTCTTTGAGAAGTCCACCGAGCACCCC37540.13967639279204327No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT37310.1388206237365779No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCTTGGAGGGACAGGTAC36070.13420691230711246No Hit
ATATAGGACATGGGCTTACCATGGAAGCTACGAGGCCCCCACACAAGGGT35500.13208609334356786No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA33060.12300749988558739No Hit
ATGCTGGATGAGGGGGTAGAACCAGATGACGTCGATTGTTGGTGCAACAC30660.11407773582855746No Hit
ATATGTACTTGGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAACC30360.11296151532142873No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATGGGCTTGGTTTCC28730.10689671723269588No Hit
ATAATATACAGATTATAGTTGGGGACTCTGATTGGCTGTAGTAGACGTGA27550.10250624990465616No Hit
CTTAATACATTTACCAACCTAGTGGTGCAGCTCATTCGGAATATGGAGGC27540.10246904255441854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTT850.093.800964145
CAACTCC3800.076.29743145
TGACGGG1350.075.1671145
ATGTTCG1950.074.4020541
TGTTCGA2050.070.7742
CGAGTGG2100.069.0927666
CGAATAA1600.067.9524145
CGAGTTT3450.065.187534
TCGAGTG2250.064.486585
AAGTTTT451.19564575E-464.42895145
TCGAGTT3450.063.083533
CTACGCC1150.063.02831145
GTAGAGG702.3399298E-762.12791145
GGCTTCT8800.055.230841
CTCGTCC1353.6379788E-1253.690784145
GCTGTTT953.148125E-853.408203145
TCTTCCG9400.052.4811025
ACAGTTC1800.052.395354
CAGTTCG1800.052.395355
CTTCCGT9650.051.121496
Produced by FastQC (version 0.11.2)