FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_extraction_neg_ctrl_rr.352000000a9a19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_extraction_neg_ctrl_rr.352000000a9a19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47050
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCGGCGCAGGCGTCGCTGCACCTGCCGCTGTGTTCGC1220.2592986184909671No Hit
GTAGAAACACGAGCACGAACACGAGCACGAACACGAATGCGAATGCGAAT1000.21253985122210414No Hit
ATCTGCAACAGATCAAAGAAACAAAAGTGAAAACATGGAAGCCAGGGATG780.16578108395324123No Hit
TTACAGAGCAAGTCAAAACCAACTGGGACCCAACAAGAAAAAAACAGAAT730.155154091392136No Hit
ACATAGAAAGTGAAACTGTGGGCCGGGCACGGTAGCTCATGCCTGTAATC690.14665249734325186No Hit
CTGTAGGGTGGAGTCTGAATTCCAAGAATTGAGTTCCCCATCTGTTAAGC670.14240170031880978No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN660.14027630180658873No Hit
GTTGCAGATGGTAGTGTTTGTTAACTCTGTCTGGTTTCCTGGTTTCATTG650.13815090329436772No Hit
GTACGAAGTGCTTCATTTCCTGTAAACTAGTTAATACATTGGCTGTGGTG620.13177470775770456No Hit
GTCTTATGCAGGAATTTGGAGGTGACAGTAATAGCAGGATTACCGTAAAC620.13177470775770456No Hit
GTTTACGGTAATCCTGCTATTACTGTCACCTCCAAATTCCTGCATAAGAC610.12964930924548354No Hit
AGTTAATACATTGGCTGTGGTGATGACTATGGGAACACTGGGGATTTTTT610.12964930924548354No Hit
GTCAAAGAACAGATATCGAACTACATAGAAAGTGAAACTGTGGGCCGGGC570.12114771519659936No Hit
CTCTATGACAGAAATGTCACGTCCTTCTTTTCTCCACCTTCTCCTTTCCC560.11902231668437832No Hit
CTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAGAGACACTA560.11902231668437832No Hit
ATCCAGGACAGGGTCCCTCCCCCTTGAGGTACCTGCATTTATTTCCCCAT540.11477151965993623No Hit
ATAATATTCCTACCTGTGACTAATTTGGTGTCTGTAGGGTGGAGTCTGAA540.11477151965993623No Hit
GGATATGAGAGTCCTGGACGACAGAATCCCGTGGAGTCTTGATCTCTCAT530.1126461211477152No Hit
GTATAGAGGGTGGCAGGTACCCAGTAGTAACTGTAGAAGGGAAATAGACT510.10839532412327312No Hit
GTATTAACTAGTTTACAGGAAATGAAGCACTTCGTACTGTGCATTTGCAT500.10626992561105207No Hit
ACCGTAAACCTTGCAAACATAAGGATTATCAAGACAATTTCTTCTGACTC500.10626992561105207No Hit
CCACTAGAGAGTCAGAAGAAATTGTCTTGATAATCCTTATGTTTGCAAGG490.10414452709883104No Hit
ATTTCATACTTAACATGAAGTATCTCAACGCTTTACAGAGCAAGTCAAAA490.10414452709883104No Hit
GGTGATGACTATGGGAACACTGGGGATTTTTTCCCCCCAAAATTGATTGT480.10201912858660998No Hit
CTCTAGTGGAGAATTTTAATTCAGGGCTAGTGCAGAATCATTTTTTTCTG480.10201912858660998No Hit
TGTTAACTCTGTCTGGTTTCCTGGTTTCATTGGGGACTTGGTGATTCACT480.10201912858660998No Hit
ACCCTACAGACACCAAATTAGTCACAGGTAGGAATATTATAGATGTTAAC480.10201912858660998No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGAGT100.0070695058145.0145
TGCACGT203.842897E-4108.752
GTGCACG203.842897E-4108.751
CACGTGT300.001924231872.54
ACGTGTT300.001924231872.55
TAGGTCT300.001924231872.54
AGTAACA754.7220965E-967.666673
GCACGTG350.003545374162.1428573
TTTAGGT350.003545374162.1428572
ACGAGCG606.7054334E-660.4166689
AGAAGCG400.00601517154.3755
AGCGGTG400.00601517154.3758
AAGCGGT400.00601517154.3757
TATGTGT553.1874556E-452.7272724
AGAGGAT553.1874556E-452.7272723
GTAACAA1004.575668E-850.754
GAGGATT604.8967596E-448.3333324
GTGTATG604.8967596E-448.3333327
GAAGCGG450.00958245448.3333326
GCGGTGC450.00958245448.3333329
Produced by FastQC (version 0.11.2)