FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_dakar-41671_rr.352000000a9b32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_dakar-41671_rr.352000000a9b32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013037
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGGTTTTGGCGATACTAGCCTTCTTGAGATTCACAGCAATCAAACCA47240.2346703016387677No Hit
ATTCCACACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTTCCAT27830.13824882503401578No Hit
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTTGCCA27800.13809979647666684No Hit
CCATAAGTCCTCCCTTTTTCCCAAATAGGGAATGTCTGTCCATTTTGTTA23990.11917316969335388No Hit
GATATATATCAACAAGGGTTGAAATGGGCGAGGCGGCTGCTATCTTCATG21620.1073999136627891No Hit
GTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGA21330.10595930427508286No Hit
CTCTATTCCACACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTT20950.10407160921532987No Hit
GGCTTTTTCCGTGCCATGGCGTTATCGGCCTGACTATGAGCTTGGTTTCC20580.10223359034135984No Hit
CATCAATAGATGGGGTTCCGTGGGGAAGAAGGAGGCTATGGAAATAATAA20260.10064395239630468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTT2750.065.952334
AGTCGCC1650.065.891655145
CGCGGCC1051.6370905E-1162.126415145
TAATTCC1051.6370905E-1162.126415145
TTCGAGT3000.060.4517822
TGTTATT851.3089448E-859.690086145
CGGTTCC1154.0017767E-1156.72412145
GCGACAG805.8551814E-754.40391
TTACGAC400.00605811154.36061145
GTTCGAG3350.054.1332321
TCTACCC1258.913048E-1152.186188145
CAGTTCG1301.3096724E-1050.2189871
AAGCGAG5100.049.785067
TCCAGGG2200.049.41874145
GAAGCGA5200.048.830096
TGAAGCG5100.048.365045
CGACAGT901.3189565E-648.361432
TGGCGGG1700.046.899353145
TATCGGG657.34793E-444.60358145
ACAGTTC1002.7232145E-643.5285344
Produced by FastQC (version 0.11.2)