FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_dakar-41667_rr.352000000a9b25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_dakar-41667_rr.352000000a9b25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583589
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGGTTTTGGCGATACTAGCCTTCTTGAGATTCACAGCAATCAAGCCA53880.2085471024996623No Hit
ATTCCATACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTTCCAT47530.18396888978858478No Hit
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTTGCTA35330.1367477567058847No Hit
CTCTATTCCATACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTT34480.13345775972881135No Hit
GTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGA29920.11580789359298249No Hit
GGCTTTTTCCGTGCCATGGCGTTATCGGCCTGACTATGAGCTTGGTTTCC28600.11069872181682149No Hit
CATCAATAGATGGGGTTCCGTGGGGAAGAAGGAGGCTATGGAAATAATAA26860.10396390447551837No Hit
TATTTACACAGTGACAAGAAATGCCGGTCTGGTTAAGAGACGTGGAGGTG26830.10384778693515107No Hit
GTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGAA25920.1003255548773431No Hit
CTATAAGGGATCCACACCATAAGTCCTCCCTTTTCCCCAAATAGGGAATG25850.10005461394981942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTT4850.083.763644
TTCGAGT5000.082.7000352
GTTCGAG5600.076.425731
TGTTATT1100.065.89405145
ATTAATT808.107236E-963.423023145
TCCAGGG2650.062.910168145
CAGTTCG1650.061.548811
TGACGGG502.0131122E-457.986763145
AGTCGCC1900.057.22378145
GAATTCC1051.1132215E-955.22549145
AGTTCGA1850.054.8980752
CTAATTT805.8866317E-754.36259145
AGCGCCC953.147761E-853.408863145
CGACAGT1101.6698323E-952.756121
CGATAGG553.2235874E-452.715237145
TTAGGCC701.6990323E-551.773895145
TCTACCC1056.924711E-848.322304145
TACGTTT450.00964954748.322304145
CGACCCC1255.2132236E-946.389412145
TATGGCC1109.985524E-846.125835145
Produced by FastQC (version 0.11.2)