FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_472_rr.352000000a9a0c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_472_rr.352000000a9a0c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802641
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT78580.979018016772131No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT33680.41961474681707017No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC23150.28842284408596125No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT20600.2566527251909633No Hit
GTATGCAGAAACCAACCTGCTGCAACGGCACTGAATACAACAATAACCTC14160.17641760139340004No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTCCCTTGATAAAG13110.16333578773075386No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT12650.15760470745949934No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT12200.15199821588979381No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA11800.14701466782783337No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC11740.1462671356185393No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC11480.143027829378265No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA11440.14252947457206896No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT10740.13380826546363817No Hit
CGATAGAACAAAAATTAGACAGCTGCCTAATCTTCTCCGAGGATACGAAC10590.131939434940403No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT10410.1296968383125208No Hit
GGGATTTTGAGGATGAAGTAACCGCCCTTTGTATTCAGGGTCCATTAGAT10380.12932307220787376No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10310.12845095129703069No Hit
GAACAAAATAGTACCAAATTGAGTACTATTTCCTTTTATTCATCCATTAT10190.12695588687844253No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC9730.12122480660718801No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT9480.11811008906846274No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT9280.1156183150374825No Hit
CCATTCATTGAACCAGTATACACTCTGTATCACCCAAGGGTTGTTGCTAA9270.1154937263359335No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC9260.11536913763438449No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC8600.10714628333214973No Hit
AACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTATTCAAT8550.10652333982440468No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA8540.10639875112285566No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAACCTGAGACCAC8270.10303485618103236No Hit
ATTCAATATCTGCGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA8150.10153979176244422No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC8100.10091684825469917No Hit
GGATACAACTTAAAACGAATGCTGAAGACATAGGAACCAAAGGCCAAATG8070.10054308215005213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA10300.0116.2237854
CGGTGCA2150.0104.60962
CAGCATC650.0100.456354
GAAACAC5300.099.929864
TAGAAAC5850.099.216162
AAACACG5350.098.995945
GAGCATC4500.096.7357564
TAGTAAC12550.095.386852
AGCATCT5250.095.353815
ACGGGAA406.1312494E-790.587975145
TTTCGGG259.401313E-486.96446145
TTCGGAA502.2744644E-886.96446145
TGAGCCC259.401313E-486.96446145
CACGTGC1200.084.643781
TAACAAG14150.084.601065
ACGTGCA702.910383E-1182.916362
GAGCAGA1450.080.0571751
GCGGAGC1050.076.006671
AGTAACA15800.075.7661363
ACAAGAG15800.075.7614067
Produced by FastQC (version 0.11.2)