FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_468_rr.352000000a99fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_468_rr.352000000a99fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068031
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT74240.6951109097020591No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC26480.24793287835278188No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT25070.23473101436194266No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT23840.22321449471035953No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA22670.2122597565051951No Hit
TAATATATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACC22240.20823365613919448No Hit
GGTGGATTAATACCTTTTTGCCAGGATATCATTGATTCATTAGACCGACC20910.1957808340769135No Hit
GGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCTGAATTGCAACCAG17950.16806628272025811No Hit
CAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTT17390.16282298922035035No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT16740.15673702355081454No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA16360.15317907439016284No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTTCCTTGATAAAG15600.1460631760688594No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC14830.13885364750648624No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC13000.12171931339071619No Hit
GGGATTTTGAGGATGAAGTAACCGCCCTTTGTATTCAGGGTCCATTAGAT12960.12134479242643706No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12460.11666328037294799No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT12250.11469704531048255No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT12160.11385437314085452No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA12080.11310533121229628No Hit
GGATACAACTTAAAACAAATGCTGAAGACATAGGAACCAAAGGCCAAATG11620.10879834012308633No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT11460.10730025626596981No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA10960.10261874421248073No Hit
GCCAAAAACACAATGGCAGAATTTAGTGAAGATCCTGAATTGCAACCAGC10830.10140155107857356No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT10770.10083976963215487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCG100.007095419144.9693145
TCGAGAA100.007095419144.9693145
AGCACGC650.0133.911961
TGACGCC351.904482E-9124.25941145
GCACGCA750.0116.067922
CGGTGCA3600.0112.84382
GTAACAA12500.0102.7345664
CACGCAC850.0102.412873
TTCGGAA607.2759576E-1296.6462145
GAAACAC5850.094.256454
GAGCGTT700.093.281984
AAACACG6000.091.900035
ACGCACT950.091.645464
CAGAGCG651.6370905E-1189.283012
ACGTGCA1150.088.3125462
AGCGTTT750.087.0631945
GAGCATC5950.086.575444
GAGCAGA2100.086.351951
TAGAAAC7000.084.9782942
CGCACTT1000.079.807925
Produced by FastQC (version 0.11.2)