FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_463_rr.352000000a99e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_463_rr.352000000a99e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463094
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT21400.462109204610727No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT14460.31224762143322954No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC10480.22630394693086067No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT8560.1848436818442908No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT7600.16411354930100586No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTCCCTTGATAAAG7010.15137315534211196No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC6480.1399283946671734No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT5850.12632424518564264No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC5720.12351703973707281No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT5610.12114171204982142No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC5610.12114171204982142No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC5550.11984607876586612No Hit
GAATGGATGAAGAACACCCAGGTCTTTTTCAAGCAGCCTCACAGAATGCT5320.11487948451070408No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT5200.11228821794279348No Hit
ACATCAGCTGAGTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTG4800.10365066271642474No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC4790.10343472383576552No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT4710.10170721279049177No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4680.10105939614851413No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA306.475602E-10145.061112
AGCACGC306.475602E-10145.061111
AGCGGTT100.0070967535144.95137145
TTCGGAA500.0130.45624145
CGGTGCA1550.0116.984762
CGCACTT404.794856E-9108.795825
GTAACAA5750.0103.4348754
GGATGCC301.5952048E-596.7074059
ACGCACT451.08721E-896.70744
GAAACAC2900.095.040034
TAGTAAC6550.091.908942
TAGAAAC3250.091.5000842
ACACGTG259.367842E-487.036667
CACGCAC502.2593667E-887.036663
CGTGCCT353.4281482E-582.892053
GAGCATC2200.082.421084
AAACACG3350.082.273465
AGCATCT2800.075.1209265
TAACAAG8050.072.981065
AACACGT300.001931850672.5305566
Produced by FastQC (version 0.11.2)