FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_460_rr.352000000a99d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_460_rr.352000000a99d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832464
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT21480.2580291760364412No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA17040.2046935362970651No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG16530.19856714524592056No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA15340.18427223279324992No Hit
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT15320.18403198216379327No Hit
GTCTATTGGAATTGAAGGGTTTGAGCCATACTGTATGAACAGTTCCTCAA14900.17898671894520365No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC14390.17286032789405908No Hit
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG14140.16985719502585098No Hit
GTATATGCATACAATAGGATATTATTCATCCATAAAAGGGAATGAAGTAT14020.16841569124911107No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG11390.1368227334755617No Hit
CTTTATATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAG11390.1368227334755617No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG11260.1352611043840935No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC11250.13514097906936517No Hit
GTGTACACTGTACCCAATGTGTAGTCTAGACAAAGATTTTAAATCAACTA11000.13213784620115704No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC10910.1310567183686021No Hit
ATACAGTGTCTGAGGAAGCCCAGATATTGGACACACTAGACAAATACTTT10900.13093659305387378No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT10690.128413961444579No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT10640.12781333487093735No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10530.1264919564089258No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG10410.1250504526321859No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT10280.12348882354071768No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG10140.12180706913452112No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT10050.1207259413019662No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA9840.1182033096926714No Hit
ATATAAAGAGATCCATATCTAGACACATTTAAATCAAAGTGTTGAAATAC9700.11652155528647486No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC9340.11219704395625517No Hit
CCCTAAGGAAGAGGAAGAATATGATTTTCAGACTTGTCATGTTATGTTAC9320.11195679332679852No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG9120.10955428703223202No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG9110.1094341617175037No Hit
GAGCCATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAA8910.1070316554229372No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA8720.10474927444309905No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA8590.10318764535163082No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT8590.10318764535163082No Hit
ATATATATTTGACTACCAAAAAAGAGATCTCTTTCAAACAACTTTCAAAA8490.10198639220434759No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAA501.9463187E-10101.48236145
GCACGCA950.099.258962
AAACACG2950.088.517765
CGCACTT1150.081.996535
AGCACGC1150.081.996531
GTAACAA12050.080.661764
AACACGA2900.075.036616
CGGTGCA1100.072.5353852
GAAACAC3700.070.5749744
TAGTAAC14550.068.297922
GCGGAGC651.3930548E-766.955741
ACACGAG3300.065.941267
CACGCAC1450.065.031733
GAGCATC1900.064.9000854
AGCATCT2700.064.475915
ACCGGAC1500.062.8640063
ACAAGAG15400.062.6441967
GCCGACC350.00356874862.13206145
ACGCTCC350.00356874862.13206145
TAACAAG15650.062.106985
Produced by FastQC (version 0.11.2)