FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_451_rr.352000000a99c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_451_rr.352000000a99c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814464
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT27390.33629479019330505No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA22530.27662364450730786No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG22330.2741680418041804No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT19710.24199964639321073No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA19000.2332822567971083No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC18010.22112702341662735No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG15270.18748526638378124No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC14510.1781539761118969No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG14350.17618949394939493No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT14180.1741022316517366No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT13890.17054160773220178No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC12670.15556243124312433No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA12530.1538435093509351No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG12230.1501601052962439No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC12180.14954620462046206No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC11820.14512611975483264No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG11720.14389831840326892No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA11220.13775931164545027No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC10670.13100640421184975No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA10660.13088362407669338No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT10550.12953304258997328No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT10470.1285508015087223No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT9990.12265735502121641No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA9900.12155233380480904No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG9860.12106121326418356No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC9770.1199561920477762No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG9670.1187283906962125No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9660.1186056105610561No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG9620.11811449002043062No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT9290.11406274556027031No Hit
GGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATG8800.10804651893760804No Hit
GAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG8750.1074326182618262No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT8400.10313531353135312No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG8400.10313531353135312No Hit
GATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTG8330.10227585258525854No Hit
AAATACAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGA8250.10129361150400754No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG100.0070751123145.106231
GCACGCA2550.0113.815812
AGCACGC2700.0107.48611
CGCACTT2700.0104.811845
CACGCAC3250.091.539813
TTCGGAA502.2730092E-886.972786145
GTAACAA15600.086.516274
TAGTAAC16450.083.364032
ACGCACT3750.077.399514
TAACAAG19200.069.916555
AAACACG3350.069.3135
CGGTGCA1900.064.919942
AGTAACA21150.064.156513
GCCGACC350.003570686362.123425145
CCCGAAA350.003570686362.123425145
AGAAGCG1650.061.5677955
TAGACGT651.0879352E-555.8169566
GTAGTAA24700.055.8100931
ACAAGAG24450.055.1971747
AACACGA3900.053.9563876
Produced by FastQC (version 0.11.2)