FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_450_rr.352000000a99ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_450_rr.352000000a99ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788874
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT42470.5383622733161443No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA32850.4164163098289461No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG32580.4129937100221328No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA27500.34859812847172045No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC27030.3426402695487492No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC22450.2845828357887318No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT21580.27355445863344463No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG21160.2682304144895129No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT21090.26734307379885763No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC20640.2616387407875022No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG20370.2582161409806889No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA19790.2508638895438308No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA18680.23679320144915414No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT18590.23565233484688303No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG18160.2302015277471434No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG18050.22880713523325652No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC17570.2227225133544774No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG17320.21955343945927994No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA16820.21321529166888503No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT16780.21270823984565346No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT16580.21017298072949545No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA16500.2091588770830323No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC16340.20713066979010591No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT16110.20421512180652424No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG16070.20370806998329266No Hit
ACACCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGA15190.19255292987219758No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG14200.18000339724721565No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC14040.1779751899542893No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGT13940.1767075603962103No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC13140.16656652393157842No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT13120.16631299801996263No Hit
CTGCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAA13080.16580594619673106No Hit
AAATACAATTGGACCGATTACCCCTCAACACCAGAGAGGTGCCTTGATGA12830.16263687230153356No Hit
GAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG12470.15807340589244923No Hit
CTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATGATAATGAT11640.14755208056039368No Hit
TTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCA11630.14742531760458577No Hit
ATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTG11590.14691826578135417No Hit
GAGCCATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAA11390.1443830066651962No Hit
CTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCA11330.14362242893034882No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT11270.14286185119550143No Hit
GATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTG11220.14222803641646195No Hit
GTTTAGTCACTGGCAAACAGGAAAAATGGCGAACAACAACATGACCACAA10980.1391857254770724No Hit
AGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGA10910.1382983847864171No Hit
GGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGTCAAGAATAAA10870.1377913329631855No Hit
GTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTTAC10860.13766457000737758No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT10740.13614341453768283No Hit
TTCCTCAAACACCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATT10740.13614341453768283No Hit
AATAGCAACTGTACGAAATACAATTGGACCGATTACCCCTCAACACCAGA10480.13284757768667746No Hit
GAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGAACAA10440.13234052586344588No Hit
GGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATGACCAGAG10360.13132642221698268No Hit
GGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGAACAACAACATGA10300.13056584448213532No Hit
GTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATGATA10140.1285376371892089No Hit
GTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACCAGTC10130.12841087423340103No Hit
GCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCCCGGTCAAGAC9990.12663619285209043No Hit
ACATAGAGGAAGAACCAGAGGATGTTGATGGCCCAACTGAAATAGTATTA9960.12625590398466674No Hit
CCTATTTATGAATCCGTCTGTTGGAATTGAAGGGTTTGAGCCATACTGTA9830.12460798555916407No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG9830.12460798555916407No Hit
ATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTC9800.12422769669174039No Hit
ATTTATGAATCCGTCTGTTGGAATTGAAGGGTTTGAGCCATACTGTATGA9760.12372064486850878No Hit
CATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTCA9740.123467118956893No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9670.1225797782662377No Hit
AGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGAC9660.1224530153104298No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGTTTGACA9650.12232625235462191No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG9560.12118538575235083No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC9530.12080509688492712No Hit
ATTTCATACAATGTTTCTAATAATGATAATGATACAACCATATCAGCTAT9530.12080509688492712No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT9460.11991775619427184No Hit
GCTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATC9410.11928394141523234No Hit
ACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAG9340.11839660072457707No Hit
TTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTTAG9110.11548105274099539No Hit
ATTCTGGAGTGCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCC9090.11522752682937959No Hit
ATCTATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAAT8930.11319931953645322No Hit
CAATTGGACCGATTACCCCTCAACACCAGAGAGGTGCCTTGATGACATAG8920.11307255658064533No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT8900.11281903066902951No Hit
CCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGATGG8890.11269226771322162No Hit
GATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTTACCAA8890.11269226771322162No Hit
GGTGATAATCGGTGCTCTTGACCAAATTGGGATAAGACTCCCACCGCAGT8840.11205845293418215No Hit
GATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCC8800.11155140111095055No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCCTCTGGTTCTTCCTCTA8780.11129787519933475No Hit
GAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGAAGTAAACACTCAGA8780.11129787519933475No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAACAGACGGATTCATAA8610.10914290495060049No Hit
GTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGGTC8560.10850909017156099No Hit
CTTCTGGTGATAATCGGTGCTCTTGACCAAATTGGGATAAGACTCCCACC8520.10800203834832939No Hit
ATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGC8470.1073682235692899No Hit
GAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTT8440.1069879347018662No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC8430.1068611717460583No Hit
TGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGA8280.10495972740893983No Hit
TTATGAATCCGTCTGTTGGAATTGAAGGGTTTGAGCCATACTGTATGAAC8180.10369209785086085No Hit
ACTCTGCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAA8110.10280475716020557No Hit
GTGGAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTT7950.1007765498672792No Hit
TATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTG7950.1007765498672792No Hit
GTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACAC7910.1002694980440476No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGC100.007082037145.058478
CAGGTAT151.2279334E-4145.058471
GCTACAG100.007082037145.058473
CGCACCC100.0070981793144.94804145
AGACGCC404.825779E-9108.71104145
CACGCAC501.9463187E-10101.540923
GCACGCA501.9463187E-10101.540922
CGCACTT607.2759576E-1296.705645
GAGCAGA1850.094.091981
AGCACGC554.1472958E-1092.309931
GTAACAA11500.085.77374
AAACACG2450.079.930185
ACGCACT651.5588739E-978.10844
TAACAAG13300.074.165235
ACGTGCC300.001932496172.5292362
GAAACAC2700.072.5292364
TTCGGAA502.3107004E-672.47402145
GTTGCCC702.8194336E-972.47402145
TAGTAAC13850.071.743722
CAGCATC754.838512E-967.693954
Produced by FastQC (version 0.11.2)