FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_445_rr.352000000a99ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_445_rr.352000000a99ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141911
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA76000.3548233329956287No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC44140.20607765682140855No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG34680.16191148931958424No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT34390.16055755818052198No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT31960.1492125489807933No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC31270.14599112661543828No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT27260.12726952707185313No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC25420.11867906743090632No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA25010.11676488892395623No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT24410.11396365208451704No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT23830.11125578980639252No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA22980.10728737095052035No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTTATTGAATTAATGGAT22280.10401926130450799No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT21740.10149814814901274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATTG9300.0122.466015
TATCGGG301.1037264E-7120.80422145
CGGTGCA6350.0110.8093952
ACGTGCA1950.0100.440242
GTAACAA26550.093.992414
GAAACAC10550.092.140634
TTCGGAA1300.089.20927145
GAGCATC9000.087.8583154
GAGCAGA3400.087.472861
GCTCGGA259.3675853E-487.04822
AGCATCT10900.083.85775
CGTGCCC353.4422865E-582.83717145
GGCGCTC353.4422865E-582.83717145
AGCACGC1150.082.000011
GGATTGA14200.079.695756
CACGCAC1200.078.587023
GCACGCA1200.078.585182
CACGTGC3050.076.1059341
GCGGAGC2550.073.96081
CGGAGCA2000.072.540172
Produced by FastQC (version 0.11.2)