FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_443_rr.352000000a9991.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_443_rr.352000000a9991.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687072
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT35570.21083866011646213No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA31030.18392813110525216No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG29490.17479988998691223No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA24680.14628895506534398No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT22880.13561958232962198No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG22270.13200385045807175No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC21420.1269655355550919No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA21130.12524658105878114No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT19570.11599979135448872No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA19450.11528849983877393No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT19300.11439938544413042No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC18080.10716792170102996No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG17820.10562679008364788No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC17810.10556751579067164No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA17020.10088484664554923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT2550.0105.2718355
ACGCACT2650.0104.0371254
GTAACAA26350.092.514384
AGCACGC2950.090.994981
CACGCAC3050.090.390233
GCACGCA3050.090.390232
CGGTGCA2700.088.672282
TAGTAAC30300.080.690982
TAACAAG31950.076.2990345
AAACACG9150.075.327425
AGCGATC300.001930452772.552215
GCGATCC300.001930452772.552216
ACGTGCA2300.069.39572
AGTAACA35450.069.377913
ACAAGAG36900.066.2604147
GGTGCAT3450.060.98413
AACACGA10550.059.8297846
GAAACAC11800.059.0255244
GCCGACC1154.0017767E-1156.72103145
ACGAGCG651.0893131E-555.8093874
Produced by FastQC (version 0.11.2)