FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_436_rr.352000000a9984.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_436_rr.352000000a9984.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772696
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT38480.21707049601285272No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA35450.19997788678938747No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT31500.17769544242216376No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC31400.17713132990653785No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG30960.1746492348377838No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC26590.14999751790493124No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA23850.13454083497678113No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG23650.13341260994552928No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG22680.12794071854395792No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT21220.11970467581581953No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC21130.1191969745517562No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA19780.11158145559080632No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG19080.1076326679814249No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT18490.104304404139232No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG18050.10182230907047796No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC17990.1014838415611024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA28100.097.5924454
CGCACTT4350.095.064065
TAGTAAC30450.090.0555952
TAACAAG35900.076.388525
AGCACGC5650.074.470751
CGGTGCA5100.072.544782
TGACGCC1800.068.450676145
ACAAGAG41750.065.6849757
AGTAACA42050.065.214513
GCACGCA6850.060.3657262
AACACGA9800.054.7818036
ACGCACT7450.054.5333864
AAACACG11700.052.706455
GCCGACC1301.3278623E-1050.17651145
CGACGAA2550.048.318123145
TAGAAAC20000.047.1541062
GTAGTAA61900.046.409911
AACAAGA59850.046.1840026
GAGCATC7500.045.4639634
GCGTAAG657.315837E-444.642941
Produced by FastQC (version 0.11.2)