FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_434_rr.352000000a9977.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_434_rr.352000000a9977.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261120
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC35250.27951344836335956No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT29230.23177810200456736No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA24700.19585765034255265No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC20840.16524993656432377No Hit
GGATACAACTTAAAACGAATGCTGAAGACATAGGAACCAAAGGCCAAATG18890.14978749048464857No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC16860.1336906876427303No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT16490.130756787617356No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT16290.12917089571174828No Hit
CGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTCCGAGGATACGAAC14300.11339127125095153No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC14250.11299479827454961No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13710.1087128901294088No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT13510.10712699822380106No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC13400.10625475767571681No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT13030.10332085765034256No Hit
GCACTATACCTTTTCCTTTTCACTACTTTAACTGGTTTCCCTTGATAAAG12690.10062484141080943No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT12680.10054554681552905No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT12640.10022836843440752No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAGA100.0070774877145.093089
CCGACGG100.007095155144.97214145
GTAACAA15850.0112.6003654
CGGTGCA4450.0107.5928342
TTCGGAA900.0104.7021145
GAAACAC6350.098.255914
AGCACGC607.2759576E-1296.721031
TAGTAAC20400.089.6127552
AAACACG7200.086.656255
GAGCATC6200.086.591244
GAGCAGA2750.084.411091
ACGTGCA1550.084.244252
CGCACTT702.910383E-1182.913625
TAGAAAC8400.081.17982
AGCATCT8000.079.804375
GCACGCA755.4569682E-1177.3799062
TAACAAG23250.076.449935
CACGTGC2100.075.9950941
ACAAGAG23850.074.830857
ACGCACT955.456968E-1268.731034
Produced by FastQC (version 0.11.2)