FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_428_rr.352000000a996a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_428_rr.352000000a996a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781333
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC9280.11877138172840518No Hit
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT8800.1126280343976256No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8520.1090444151213375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA16900.0100.4264761
TAGTAAC16850.0100.294032
CGCACTT6600.094.515155
GTAACAA19650.086.377424
TAACAAG22250.075.957885
GCACGCA9050.068.923762
AAGAGCG3950.064.2714169
TCTCGCT4700.061.727862
TCGCTCT4750.061.0820054
AGTAACA28050.060.768983
ACGCACT10750.059.377394
CTCGCTC5100.056.8901023
ACAAGAG30600.055.9419367
CGCTCTG5250.055.264675
GCCGTTC1353.6379788E-1253.7295468
AGCACGC11550.053.3772281
TCTGTCG5500.052.7526448
GTCTCGC5700.050.898411
AACAAGA34650.048.775266
AGCCGTT1501.0913936E-1148.3565867
Produced by FastQC (version 0.11.2)