FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_425_rr.352000000a995e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_425_rr.352000000a995e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245541
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT31730.2547487397042731No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA25710.20641632832640597No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG24070.19324935911383087No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT23630.18971675761777412No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA21990.17654978840519903No Hit
ATATTGTAGTTGGGTCTCTACAGTGAGGCAGGATCTGCCCAGGCAGAGGG19310.15503303383830802No Hit
GTAGTAACAAGAGCGTACATCAGAATCCCAGGAGGGATGGCGTGCTGGAC18840.15125957314933833No Hit
GTAGAAACACGAGCGTACATCAGAATCCCAGGAGGGATGGCGTGCTGGAC18050.14491694773596372No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG16650.1336768520666923No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG16250.1304653961611862No Hit
CCTAAGGGGTAGCCACTACTCAGCACCCACTTTTTGTTGCCAGACCTTCC16230.13030482336591087No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC15990.1283779498226072No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT15540.12476506192891282No Hit
CATTTAGGTTTCTGGTCTCTGATAATTTGGAAGGTCTGGCAACAAAAAGT15170.12179446521631966No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15000.12042959645647956No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA14950.12002816446829129No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT14760.11850272291317589No Hit
CCCATATTCCAGGCCCTCCCACAGCCCTCACCCGGTCAGTCACCCCATCT14710.11810129092498761No Hit
CAGTAAAGTCTGAGCTAAAATCGGAGGCTCTCGAGCCACATCCACCTCGC14630.11745899974388639No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG14610.11729842694861109No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGAAATAGTATGTTATCATC14120.11336439346436608No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC13520.1085472096061069No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG13520.1085472096061069No Hit
CCCCTTAGGTGGACCCTAAACTCTTCACTATAACTTCCCTGCCCAGCACC13520.1085472096061069No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA13420.10774434562973037No Hit
TCACAGAGCACCCCCTAGTCTTGGAAACCCTTCCTCAAAGAGTCACCTGG13140.10549632649587608No Hit
CTACAGTGAGGCAGGATCTGCCCAGGCAGAGGGAAGAGCAGGCAGAGCCG12870.10332859375965947No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG12600.10116086102344284No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC12560.1008397154328922No Hit
GTCCAGAAGAGGGTGAAAGGAACCTCCACTTCGGCCATTGTGGAGGCTTA12510.10043828344470396No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA12460.10003685145651567No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA2400.087.6498642
GTAACAA16950.083.029314
CGCACTT2700.077.917265
AGCACGC2800.075.1284561
AAACACG10300.072.5436555
TAGTAAC22000.067.2623442
ACACGAG10100.066.076717
GAAACAC11600.064.4137654
AACACGA10600.064.331166
TAACAAG21850.064.077465
ACGAGCC1650.061.5497179
ACAAGAG23500.059.8846977
CACGAGC10900.059.230518
AGTAACA24850.058.67483
TAGAAAC18500.055.2855832
AACACGT1850.054.89796
CGCATAC400.006038759454.4033661
GTAGAAA18950.054.355521
ATTAGTA7600.053.4489171
ACGAGCG6900.052.565759
Produced by FastQC (version 0.11.2)