FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_421_rr.352000000a9941.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_421_rr.352000000a9941.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940257
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGCTTGTGCAAAGGCCCTGAGGCATGAATGATTAAAAGATATAAT16080.17101707299174587No Hit
GTTATAACCTGTGTTTGAGAAAGGCTAGCTCTGTTTCTATTGCCATAATT14480.1540004488134627No Hit
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT12770.13581393172292258No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC11110.11815918413795377No Hit
ACAACAGCCAACATAGATACCACAGGGCACAAAAGGCCAGGAATACAAAA10980.11677658342346828No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10660.11337325858781164No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA10530.11199065787332611No Hit
GTGCTACTCCTGTGACACGCAATGTCTTCTAGCTTGTTGGTTCCACAGTT9880.10507765430089859No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC15750.0113.286842
GTAGTAA16650.0107.5988541
CGCACTT6300.0103.616015
GTAACAA17800.0101.4622044
TAACAAG19400.091.598695
CGACCGG353.4298384E-582.892815
GCACGCA8550.077.196962
TCTCGCT5900.076.219242
TCGCTCT6050.075.5283664
CTCGCTC6100.073.7202453
AAGAGCG5350.071.853359
GTCTCGC6500.069.183621
AGTAACA26350.069.0904543
AGCACGC9600.067.998011
GTGCTAC3300.065.937461
CGCTCTG7400.063.7098465
ACGAGCG1400.062.169619
ACGCACT11450.057.6448944
CGAGCTA1900.057.2614865
ACAAGAG32200.055.4149937
Produced by FastQC (version 0.11.2)