FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_420_rr.352000000a9934.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_420_rr.352000000a9934.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459326
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACAAT23740.16267783894756896No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT21480.14719123759872707No Hit
ATATTTGCTTTCTCCCCTGGTTTTAGCTTTTCAAGTTCTTCAATGGTTTT21000.1439020479317164No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT20540.14074990783416455No Hit
GCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACAAT19840.13595317290310732No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC19730.13519940027108404No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT18880.12937479356908602No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA18770.12862102093706274No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG17470.1197127989222422No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT17340.11882197672076014No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16840.11539573748429069No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC15290.10477439585123544No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA100.0070765074145.100577
TACGCGC203.8715897E-4108.71345145
CGAGCGG301.602379E-596.63417145
TACGCGG554.1654857E-1092.241714145
CGCACTT2300.091.473315
CGGTGCA5900.086.07072
AGCACGC2700.080.605891
GCACGCA2900.075.046872
AAACACG8800.071.723395
GAAACAC9000.068.5173654
GGTGCAT6800.067.2133943
CTCGCTC1100.065.9525453
GCGGTGC10750.065.459491
GTCTCGC1009.094947E-1265.290781
ACGCACT3500.060.1110274
GTAGTAA28850.060.098191
TAGTAAC29100.058.8339962
GTAACAA28950.058.6396684
CGCTCTG1251.8189894E-1258.0382355
AACACGA10650.055.8583956
Produced by FastQC (version 0.11.2)