FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_418_rr.352000000a9927.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_418_rr.352000000a9927.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4374610
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA113570.2596117139585014No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT104540.23896987388590069No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG92460.2113559837334071No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC74630.17059806474177128No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG71260.16289452088300443No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC71200.16275736579946554No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA68660.15695113392965315No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC64490.1474188556237013No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT63260.14460717641115436No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG59040.13496060220225345No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG55580.12705132571817832No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT55490.12684559309287No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT53130.12145082647367422No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG53000.12115365712600666No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA52780.12065075515303078No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT50650.1157817496874007No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC49000.11200998489008163No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC48770.11148422373651594No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC47510.10860396698219957No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT47250.10800962828686443No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG47170.10782675484214592No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG47050.10755244467506818No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA46760.10688952843796362No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA45630.10430644103131478No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC43790.10010035180278928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT7750.0100.159555
GTAACAA62600.094.564074
AGCACGC8500.090.459151
TAGTAAC68500.086.6259842
CGGTGCA8600.081.819862
ACGCACT9900.078.407734
TTCGGAA2500.072.48072145
TAACAAG85750.069.1191255
AGTAACA91450.065.365543
GCCGACC1900.061.0364145
ACAAGAG97250.061.0188567
GCACGCA12950.059.3774532
GAAACAC24300.056.4242554
AACACGA21200.054.4091076
AAACACG26050.053.469225
GAGCATC19150.053.414684
AACACGT4200.051.818196
AGCATCT21100.051.228795
TCGCGGG604.953414E-448.320484145
GTAGTAA133650.046.56761
Produced by FastQC (version 0.11.2)