FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_412_rr.352000000a991a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_412_rr.352000000a991a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172096
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG67730.31181863048410385No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA36330.1672578007601874No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT36330.1672578007601874No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT32750.15077602463242878No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG32390.14911863932349215No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG31040.14290344441497982No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA30100.13857582721942308No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG28960.13332744040779046No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG27260.1255008986711453No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT26530.12214008957246825No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG23720.10920327646660184No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT23330.10740777571525385No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG22310.10271185067326674No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC22230.10234354282683637No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC22180.1021133504228174No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA6350.0106.238452
AGCACGC3400.098.141211
ATTCGGG750.096.63642145
CGCACTT3750.087.053015
CGATATT259.3999156E-486.97278145
GGTGCAT7750.085.178943
ACGCACT4000.083.4258044
GCCGACC1000.079.72505145
GCACGCA4400.075.836392
GCGGTGC11150.073.5150151
GAAACAC10750.068.157784
GTAACAA38600.067.845714
AACACGA10850.065.523776
ACGTGCA1600.063.471762
ACGCATG350.003571489862.123417145
AAACACG11800.062.0928965
TATCGGG606.8209138E-660.397766145
GCGGAGC3050.059.458331
TGACGCC1600.058.887817145
ACACGAG12000.058.638527
Produced by FastQC (version 0.11.2)