FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_403_rr.352000000a98e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_403_rr.352000000a98e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346205
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC36680.27246964615344615No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT25500.1894213734163816No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA21150.15710831559829297No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC19670.14611444765098927No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG18850.14002325054505071No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT18530.13764619801590397No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA18510.1374976322328323No Hit
GATAAAGTTCTTCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAA18460.13712621777515313No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT17920.133114941632218No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT16300.12108111320341255No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA16300.12108111320341255No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT14510.10778447561849794No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14060.10444174549938531No Hit
GAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTT13600.10102473248873686No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG13480.10013333779030684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA15700.085.926834
CGGTGCA2950.081.134842
GAAACAC7550.071.0886464
AAACACG7550.071.0886465
AACACGA6850.070.941396
ACACGAG7150.068.9792257
AGAAGCG2950.066.383055
ACGCACT1750.066.3128054
GCCGACC554.065965E-665.899254145
TAGTAAC21400.063.3786932
TAACAAG21900.061.6005065
GAGCAGA4850.059.8182531
TTCGGAA851.3073077E-859.69697145
AGCACGC1850.058.8078041
CACGAGC8300.057.674168
GCACGCA2050.056.608492
GGTGCAT4100.056.608493
CGCACTT2000.054.3972245
CGGAGCA3350.054.1265872
ACAAGAG25300.053.895537
Produced by FastQC (version 0.11.2)