FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_402_rr.352000000a98d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_402_rr.352000000a98d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843962
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT35040.1900256079029828No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA29230.15851736640993686No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG27970.15168425379698713No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT27040.14664076591600045No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC25240.1368791764689294No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA23570.1278225907041468No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21950.1190371602017829No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC21530.11675945599746633No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA20680.11214981653634945No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC20560.11149904390654472No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG19800.10737748391778139No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT19620.10640132497307428No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG19390.10515401076594853No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT18640.10108668182966893No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACC100.007095863144.9691145
GAGCAGA3950.099.170881
GCACGCA607.2759576E-1296.722222
TGACGCC900.096.64607145
CACGCAC651.6370905E-1189.282053
CAGGATT3100.088.924465
CACAGGA3100.088.922043
AGCACGC702.910383E-1182.904761
AAACACG6650.080.725255
GAAACAC6900.077.800424
AACACGA6550.076.420016
GTAACAA14850.075.230444
ATGCCCG406.644837E-572.543635
ACGAGCC3200.070.276649
ACACGAG7350.067.1152047
ACACGTG808.052666E-963.4756777
CACGAGC8050.062.180268
AACGGCC350.003569992362.129616145
CGCACCC350.003569992362.129616145
TAGAAAC9350.061.291892
Produced by FastQC (version 0.11.2)