FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_398_rr.352000000a98c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_398_rr.352000000a98c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66871
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCAATGGAAATTTTAAACAAGGGGGATTAAGATCCTGAAGCACTTC1980.2960924765593456No Hit
TTATTAACTGAAGAAAAATGGGGCCCTTTACAGATTTTTTAAGATTAGGA1970.29459706001106606No Hit
AGCTTATACAATTCTGACTGAAAAATTAAAGTTGTGCAAACTTTCCACTT1890.2826337276248299No Hit
ATACAATTCTGACTGAAAAATTAAAGTTGTGCAAACTTTCCACTTGATGA1630.2437528973695623No Hit
CATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCAGAGTAACA1450.21683539950053088No Hit
GAATAAGCAGGAGTATTGCTGTGGTGGAAAAGGACCCTCTGGTGAAGCTT1450.21683539950053088No Hit
GTTTACAGCAGTGAGGAGAGAGGGAAAACTTGTGGAAAAACTGAATTACA1430.21384456640397181No Hit
TGTCAATGCAATCTTTTTTACATTATTAACTGAAGAAAAATGGGGCCCTT1320.19739498437289707No Hit
CTTTTATAAGTGGTACTACCAGCCACCTAATTCATCCCTAAAAAACTAAG1250.1869270685349404No Hit
GAGTAACACCTGGGAAAGCTTCACCAGAGGGTCCTTTTCCACCACAGCAA1220.18244081889010183No Hit
CTTCTCAACTGGTAGCTTATACAATTCTGACTGAAAAATTAAAGTTGTGC1160.1734683196004247No Hit
AATCAGGACTGAAAGGTGGATGCCCAATGATTTCCCATCAAAACTCTTGA1160.1734683196004247No Hit
GCTCATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCAGAGTA1140.17047748650386566No Hit
TTCTTTCACCCTCCAGAAAGTCAAAAAGCAAAATGCCTTGAGCATCCCAA1110.16599123685902709No Hit
GTGTTACTCTGCTCATGCTCTGGCTAACCTTCTCAAAACGCACTCATAAT1050.15701873756934992No Hit
GTATGATTCTGAAGACAAAGCACAATCAAAGCAATGGCTACCAAGAGGTG1040.15552332102107042No Hit
AATTAAAGTTGTGCAAACTTTCCACTTGATGAGTGCCGAAACCATTGTGT1040.15552332102107042No Hit
ACATAATGAACATAAAAAATGTGAACTTTATTTTTTTAACTTAAGTTCCA1010.15103707137623185No Hit
ACCTAATTCATCCCTAAAAAACTAAGGGTCCTGGGAATTTAACCATGTCA980.14655082173139328No Hit
GAAGAAAAATGGGGCCCTTTACAGATTTTTTAAGATTAGGAAACAAGAAG970.14505540518311377No Hit
GTCAGAAGGAGACAAATCAGGACTGAAAGGTGGATGCCCAATGATTTCCC940.14056915553827518No Hit
GGCTTTACCAGTATGATTCTGAAGACAAAGCACAATCAAAGCAATGGCTA940.14056915553827518No Hit
GCTAACACCACAGACTTCTCAACTGGTAGCTTATACAATTCTGACTGAAA940.14056915553827518No Hit
GTCAAGAGCAAAGATCATGGCAACATTTTGGGATGCTCAAGGCATTTTGC930.13907373898999567No Hit
AGTAGAAACAACAGCTAACACCACAGACTTCTCAACTGGTAGCTTATACA930.13907373898999567No Hit
GCCTTGAGCATCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCTG920.13757832244171614No Hit
AGCTAACACCACAGACTTCTCAACTGGTAGCTTATACAATTCTGACTGAA920.13757832244171614No Hit
GGCTAACCTTCTCAAAACGCACTCATAATGAGCAGATGTTATCATTCTTT920.13757832244171614No Hit
TCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCTGCATTTGCTTT910.1360829058934366No Hit
GGTAAAGCCATGTCCTATTGCAGTTCTTCCAAGAAGTGCTTCAGGATCTT900.1345874893451571No Hit
GCTCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAAGAATGATAAC870.1301012397003185No Hit
GTGCAAACTTTCCACTTGATGAGTGCCGAAACCATTGTGTCCAGCTCAGC860.128605823152039No Hit
GTAGAAACAACAGCTAACACCACAGACTTCTCAACTGGTAGCTTATACAA850.12711040660375947No Hit
GCATTGACATGGTTAAATTCCCAGGACCCTTAGTTTTTTAGGGATGAATT840.12561499005547996No Hit
CTTTTTTACATTATTAACTGAAGAAAAATGGGGCCCTTTACAGATTTTTT840.12561499005547996No Hit
CTCATAATGAGCAGATGTTATCATTCTTTCACCCTCCAGAAAGTCAAAAA840.12561499005547996No Hit
ATTTTGGGATGCTCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAA830.12411957350720045No Hit
GCTTATTCTCATCAAATGTAGGCAACTTTTCAAGAGTTTTGATGGGAAAT830.12411957350720045No Hit
TCTGGAGGGTGAAAGAATGATAACATCTGCTCATTATGAGTGCGTTTTGA810.12112874041064138No Hit
GATAACATCTGCTCATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCAT800.11963332386236186No Hit
GGTTAGCCAGAGCATGAGCAGAGTAACACCTGGGAAAGCTTCACCAGAGG790.11813790731408234No Hit
AAACAACAGCTAACACCACAGACTTCTCAACTGGTAGCTTATACAATTCT780.11664249076580281No Hit
AAGCAGAGCAGACCACAAACAGAATAAACAAAAGGTAAATTGTTGGACTT780.11664249076580281No Hit
ATCAAAGCAATGGCTACCAAGAGGTGGAAATAGCCCAGTCAAAGCAAATG740.11066082457268474No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN740.11066082457268474No Hit
TTTTTACATTATTAACTGAAGAAAAATGGGGCCCTTTACAGATTTTTTAA740.11066082457268474No Hit
GTTCTGAAATAACAAAATTTTATTTAAAAATGGGAAAGATTAGTTTACAG740.11066082457268474No Hit
TTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCAGAGTAACACC720.10766999147612567No Hit
GAAGGAGACAAATCAGGACTGAAAGGTGGATGCCCAATGATTTCCCATCA720.10766999147612567No Hit
CCCAATGATTTCCCATCAAAACTCTTGAAAAGTTGCCTACATTTGATGAG720.10766999147612567No Hit
GTCTTGGCTAAGGGAAGCCCAGGGAAAGGTTTTGATCTTTACTTCAGCCA710.10617457492784615No Hit
CCACAGACTTCTCAACTGGTAGCTTATACAATTCTGACTGAAAAATTAAA700.10467915837956664No Hit
CTTAAGTTCCATCAAGTTCAAGACACTTTTATAAGTGGTACTACCAGCCA700.10467915837956664No Hit
AGTGAGGAGAGAGGGAAAACTTGTGGAAAAACTGAATTACATAATGAACA690.10318374183128709No Hit
GCTTATACAATTCTGACTGAAAAATTAAAGTTGTGCAAACTTTCCACTTG690.10318374183128709No Hit
CCCTAACACATAGGTAATTAGATTAAACATAAATGGTTCTGAAATAACAA680.10168832528300757No Hit
GGTGGAAATAGCCCAGTCAAAGCAAATGCAGACCAGTCAAGAGCAAAGAT680.10168832528300757No Hit
TTCGTATTCCATGTGAGAACAGAGAGTTATATTAGTGAGAGACAGAAAGA680.10168832528300757No Hit
TGCTTATTCTCATCAAATGTAGGCAACTTTTCAAGAGTTTTGATGGGAAA680.10168832528300757No Hit
CTTTCCACTTGATGAGTGCCGAAACCATTGTGTCCAGCTCAGCGGCAGAC670.10019290873472805No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTTT100.0070731854145.020236
CGAGCTT100.0070731854145.020235
ACGAGCT259.342927E-487.0121464
GACGAAA353.4113997E-582.868706145
CGAATAA300.001926736172.510124145
AGCTAAC501.9952338E-458.00811
GCAGTTT553.195031E-452.734634145
GCTAACA604.9083575E-448.3400842
TAGTCTA657.2822487E-444.6216134
TAGGATG700.001048837341.4343535
ACCCGGG750.001472461338.6720627
GTAGAAA1251.2463219E-534.804861
GGGACTG850.002721901534.122414
AGGATGT850.002721901534.122416
CCCGGGC950.004692860430.5305778
ATATTAG950.004692860430.5305771
CCGGGCT1000.00603033229.004059
GGACTGG1000.00603033229.004055
CTAACAC1000.00603033229.004053
TGGGACT1000.00603033229.004053
Produced by FastQC (version 0.11.2)