FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_395_rr.352000000a98bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_395_rr.352000000a98bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131757
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT44230.20748143432858437No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT36010.16892169229419676No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA33460.15695972852440498No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT30920.14504467441645552No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC30270.1419955463967047No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT27150.12735973190190064No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT26640.12496733914794228No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC25830.1211676565387143No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC25330.11882217344659828No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC23830.11178572417025016No Hit
GGAACAGGCTACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAA22450.10531219083600991No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA30600.0109.77574
CGGTGCA4950.0102.590912
AAACACG13400.094.7501455
GGTGCAT5300.094.449453
GAAACAC14100.089.531694
TAGAAAC16150.081.7551042
CGCACTT1750.078.770255
TAGTAAC43400.078.229782
ACGCACT1950.078.132424
TAACAAG43200.078.093675
ACAAGAG45550.073.590327
AACACGA16550.073.209116
GCCAGCG607.996641E-872.551544
AGCACGC1800.072.544721
ACGATCC1450.069.97904145
GAGCAGA5550.066.662721
CGGAGCA5450.066.556352
GCGGAGC7050.065.85621
TTCGGAA1550.065.464264145
ACACGAG19050.063.6046077
Produced by FastQC (version 0.11.2)