FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_390_rr.352000000a9892.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_390_rr.352000000a9892.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797111
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC40170.22352542497374953No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT31520.17539261626020874No Hit
GATAAAGCTCTTCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAA27310.1519661278574334No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA25860.14389762235053927No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA24480.13621863090259867No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGCTCTTCCGTGACC23890.13293558383427623No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT21410.11913565717420904No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG21330.11869049824969075No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT20500.11407197440781343No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT19380.10783974946455728No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19190.10678249701882633No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT18150.10099543100008848No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC17990.10010511315105189No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGG100.007096536144.9644145
GTAACAA19900.094.776924
AAACACG7450.094.449095
CGGTGCA3350.080.1174552
GAAACAC9000.078.988874
AACACGA8500.074.2476356
TTCGGAA903.6379788E-1272.4822145
TAACAAG27100.069.596345
CACGCAC955.456968E-1268.720943
GCACGCA955.456968E-1268.720942
ACACGAG9700.065.064177
GCGGAGC3800.064.9031141
GAGCAGA4600.063.0771981
ACGAGCC3850.062.1795549
TGGCGCC350.003570436562.1276145
TAGAAAC12750.062.013542
TAGTAAC31700.061.5549852
CAGGATT3300.061.5497635
CGACGAA2250.061.207188145
AGCACGC1102.5465852E-1159.3499071
Produced by FastQC (version 0.11.2)