FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_385_rr.352000000a9885.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_385_rr.352000000a9885.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293503
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA41980.3245450532391498No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG39910.30854199796985393No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG38680.2990329361431709No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT31530.24375668243521664No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT30950.23927273458198398No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC30740.23764923622133075No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC29980.23177371834468105No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT29310.22659398547973988No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA27380.2116732624508795No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG26560.20533388789975748No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT26220.2027053667444142No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC26000.2010045589380156No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA25510.19721639609649147No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAA25020.19342823325496733No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT24640.19049047431664248No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG23750.18360993364530273No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA23200.17935791412930624No Hit
CTTCAAGGGACATCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTT22890.17696132131119913No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG22610.1747966568303282No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG20650.15964400546423163No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC20560.1589482204525231No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT20370.1574793409833607No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT19690.15222229867267412No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT19550.15113996643223868No Hit
GTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATGACCACA18870.1458829241215521No Hit
AAATACAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGA18610.14387287853217193No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG18190.14062588181086552No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGTTTGACA17580.1359100056203967No Hit
AATAGCAACTGTACGAAATACAATTGGACCGATTACCCTTCAACACCAGA17390.13444112615123427No Hit
ATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTC17090.13212184277887257No Hit
TTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTATGAAC16890.13057565386396475No Hit
TTCCTCAAACACCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATT16850.1302664160809832No Hit
GTTTAGGCGGTCTTGACCGGGGTAGTCAAGGGCTCTTTGCCATGAAAGCC16550.12794713270862149No Hit
GGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATGACCAGAG16440.12709672880542217No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG16390.12671018157669522No Hit
GAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACA16370.12655556268520446No Hit
CCTTTATCTTTTGCCTTGCATCTTTGTTGTTCATGTCCCTTAATACTATT16310.12609170601073208No Hit
TCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTTATTCTTGACTCT16140.12477744543306045No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG16000.123695113192625No Hit
CCTATTTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTA15880.12276739984368029No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC15870.1226900903979349No Hit
ACTCTGCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAA15820.12230354316920795No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCCTCTGGTTCTTCCTCTA15760.12183968649473562No Hit
GATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCC15670.1211439014830271No Hit
GAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGATCTTACAGTGG15630.12083466370004553No Hit
GGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATG15350.1186699992191746No Hit
CATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTCA15310.11836076143619303No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC14990.11588685917234053No Hit
GCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCCCGGTCAAGAC14860.11488183637765045No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14800.11441797970317813No Hit
CTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCA14660.11333564746274265No Hit
ATTCTGGAGTGCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCC14590.11279448134252491No Hit
CTTCTGGTGATAATCGGTGCTCTTGACCAAATTGGGATAAGACTCCCACC14470.11186676799358021No Hit
GAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG14420.11148022076485327No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC14400.11132560187336249No Hit
GGTGATAATCGGTGCTCTTGACCAAATTGGGATAAGACTCCCACCGCAGT14360.11101636409038093No Hit
GTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACCAGTC14150.10939286572972773No Hit
GCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTG14140.10931555628398233No Hit
CAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGACATAG14050.10861977127227382No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA14000.10823322404354688No Hit
GAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGAAGTAAACACTCAGA13970.10800129570631069No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG13590.10506353676798584No Hit
AGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCG13540.1046769895392589No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA13500.10436775175627733No Hit
CCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATG13440.10390389508180499No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA13370.10336272896158726No Hit
ATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTG13070.10104344558922553No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT12990.10042497002326241No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGG202.1346787E-6144.94765145
GCCGACC1300.089.198555145
CGGAGCG259.361897E-487.059545
ACGAGCG554.044885E-665.956749
GGAGCGT350.003557085862.1853836
CGACGCA350.003571844762.120422145
TGACGCC2700.059.05275145
AATGGCC753.7714744E-757.97906145
AACACGA5950.053.650136
TAGTAAC13900.051.6700252
GAAACAC7450.051.6124764
ACACGAG6250.051.0769047
GTAACAA14800.047.5494044
CCGACGC1201.9796425E-742.2764145
GCTGCCC5350.041.994183145
ACAAGAG17700.039.7603657
CACGAGC8250.037.8151978
GTAGAAA11550.037.686651
TCATAGA4450.037.49611
AAACACG10450.037.4897545
Produced by FastQC (version 0.11.2)