FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_383_rr.352000000a9878.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_383_rr.352000000a9878.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822758
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTGTGATTCTAATTCACTCACGTCATTAGCTCCCATTATGACTTCC41120.2255922069742665No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT25940.14231181539184026No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT23560.12925467889867992No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA21300.11685588542198141No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG20270.11120510786401705No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC19240.10555433030605268No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA18990.10418278235509047No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG6600.070.346335
CGCACTT1350.069.85785
AGAAGCG6050.068.347855
GTAACAA17600.065.949684
ACACGAG6950.062.6284687
AACACGA7200.061.4614336
GAGCAGA10500.058.030931
GAAGCGG7200.056.423616
GCACGCA1800.056.420512
ACGAGCC2850.055.9962969
CACGAGC7950.055.661668
CTTAGCG701.6909207E-551.8147549
TAGTAAC23050.051.2977332
GAAACAC9200.049.6773074
ACGCACT2050.049.5426834
CGGTGCA1950.048.360442
AGTCGCC450.0096493948.321926145
TAACAAG25000.046.1383975
GCAGAAG14550.042.380033
CAGAAGC14700.041.947584
Produced by FastQC (version 0.11.2)