FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_380_rr.352000000a986b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_380_rr.352000000a986b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005663
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT15090.15005026534733804No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA14530.14448179956904053No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT13040.12966570312321324No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT12770.12698090712296267No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC12350.12280455778923953No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG11750.11683834445534935No Hit
CTATAGAACATGCTGGTGAATTCAAACATTCCAGTTTCGTTGCAGTAACT11560.11494904356628413No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT11320.11256255823272807No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10720.10659634489883789No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT10240.10182337423172574No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC1350.096.728741
CGGTGCA3400.091.750052
GTAACAA20200.081.165954
GAAACAC5900.078.6945654
AAACACG5900.078.6945655
CGCACTT1600.077.080715
GCGGTGC6750.076.308221
GGTGCAT4050.075.233463
GCACGCA1900.072.546552
ACGCACT1950.070.6863864
TAGTAAC23400.070.376352
CTCGCTC851.6916601E-1068.2791063
TAACAAG24450.067.057345
GTAGTAA25450.065.8477551
ACGAGCG350.003557450662.1827555
GTCTCGC954.5292836E-1061.091831
AGTAACA27950.059.1792953
AACACGA7200.058.4402736
ATTCGGG753.7678365E-757.98525145
CACGCAC2350.055.5675663
Produced by FastQC (version 0.11.2)