FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_379_rr.352000000a9842.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_379_rr.352000000a9842.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927709
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT34720.1801101722303522No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT30170.15650702465984234No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT30070.15598827416378716No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC28760.14919264266546453No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT27320.1417226355222702No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT26580.13788388185146203No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC24400.1265751210374595No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA24020.12460386915244988No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT22240.115370110322668No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT21330.11064948080856601No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC21310.11054573070935499No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC21240.11018260536211637No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT19610.10172697227641724No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGC100.0070784874145.08828
TTCGGAA1300.0117.07405145
GAGCCCC458.54925E-11112.737976145
GTAACAA27100.0111.891684
CGGTGCA4750.0111.485922
TTCGTCC352.7685383E-7103.534874145
GGTGCAT5050.0103.426533
GAAACAC10300.0102.826834
AAACACG10500.0100.1773455
GGCCGCC301.602644E-596.632545145
TAGAAAC12500.092.8540342
GAGCAGA2800.088.084691
TAACAAG35850.084.784355
GGTACCC451.2359142E-680.52713145
TAGTAAC39950.076.990982
ACAAGAG40050.075.5328147
AACACGA13100.073.095986
GCCGACC608.0553946E-872.47441145
GCGGAGC5550.066.658681
CAGCCTT1750.066.324315
Produced by FastQC (version 0.11.2)