FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_377_rr.352000000a9835.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_377_rr.352000000a9835.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462473
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAACAACAGCTATGAGTGACTTGGCTAAGATTCACCCATGGGTCACTC20340.13907949069828981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT5700.075.097145
ACGCACT8100.058.2203184
TACGACG400.00603447554.4136056
TAGTAAC12250.050.9306072
AACACGA7750.049.6158456
GCACGCA9950.049.579522
AGCACGC10000.049.3316271
GTAGTAA14100.048.364341
ACACGAG8200.047.7728967
AAACACG11800.039.9647945
CACGAGC9950.039.3706258
CACGCAC13450.037.7591323
ACGACGG800.002030861836.272017
GTAACAA16550.035.9469534
ACGAGCA5200.033.4818539
GCCGACC2051.1435986E-831.816998145
TAACAAG18950.031.3943045
GTAGAAA25350.030.9073871
ACGAGCT3800.028.6357989
AGTAACA22750.027.1071433
Produced by FastQC (version 0.11.2)