FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_373_rr.352000000a9828.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_373_rr.352000000a9828.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3870167
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTGTGATTCTAATTCACTCACGTCATTAGCTCCCATTATGACTTCC48190.12451659062774292No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT46650.12053743417273724No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT45180.11673914846568638No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG16600.064.6748357
CACGAGC17200.061.9969988
AAACACG21050.056.860885
ACGAGCC5850.055.800549
CGCACTT7250.052.0291945
AACACGA22150.050.107126
GTAACAA38150.048.1069374
ACGCACT8300.048.0691384
AGAAGCG12150.046.5693365
AGCACGC9200.044.9413721
TGACGCC8550.044.928368145
TAGTAAC43050.044.8195722
GCACGCA9300.043.6781582
ACGAGCA13600.042.137619
GAAGCGG14650.039.117516
TAACAAG52800.035.171255
TAGAAAC45800.035.1598322
CACGCAC12150.034.6275863
TTCGTCC1301.6593236E-533.451748145
GAAACAC37750.032.4751744
Produced by FastQC (version 0.11.2)