FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_369_rr.352000000a981b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_369_rr.352000000a981b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770268
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC23010.1299803193640737No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT21290.12026427636945367No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG18780.10608563223195584No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA18240.10303524664062164No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC18090.10218791730969548No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT17800.10054974726990488No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGC100.007099544144.9438145
TATCGGG300.001938969172.47189145
TGACGCC1250.069.57302145
AAACACG7400.065.690745
CACGAGC6750.064.490588
ACACGAG6800.064.016387
ACGAGCC2500.063.8438649
GCACGCA1750.053.8941732
AACACGA8150.053.413956
AGCACGC1800.052.3956261
ACGCACT1700.051.2145544
CGCACTT1600.049.880845
TGCGCGT450.00961313548.3679358
TAGAAAC12150.046.575212
GAGCAGA8100.046.573891
GTAACAA14500.046.5346034
GAAACAC11700.042.168114
GCCGACC1401.4204488E-841.412514145
TAGTAAC18250.040.548412
CACGCAC2350.040.1362273
Produced by FastQC (version 0.11.2)