FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_368_rr.352000000a980e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_368_rr.352000000a980e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541623
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATTCTTTTAC19160.12428460135843848No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT16290.10566785783554086No Hit
ATATTTGCTTTCTCCCCTGGTTTTAGCTTTTCAAGTTCTTCAATGGTTTT16110.10450025719647411No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA16080.10430565708996298No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG6000.065.29015
AACACGA5950.059.7425776
ACGAGCG851.3003955E-859.7386939
ACACGAG6150.057.7997257
CACGAGC6400.055.5419248
CGCACTT3550.053.1312265
AGCACGC3950.049.5841941
ACGAGCC2650.049.2723469
ACGCACT4050.048.3630374
GTAACAA22350.042.520524
AAGCGGT3250.042.4106647
GTAGTAA23400.041.849911
TAGTAAC23000.041.3175132
GCGGTGC3650.037.7604689
TGACGCC1752.4374458E-937.27471145
AGAAGCG4650.035.882255
GAAGCGG4700.035.5005266
TAACAAG27350.035.0123635
GCACGCA5700.034.3620952
AGTAACA27900.034.321043
Produced by FastQC (version 0.11.2)