FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_366_rr.352000000a97f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_366_rr.352000000a97f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504321
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT28590.19005252203485826No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT3500.064.24735
AGCACGC4350.055.0264431
ACGCACT4600.053.6145024
TAGTAAC21950.050.890062
GTAGTAA23500.049.3854331
GTAACAA23250.048.6701744
AACACGA8650.046.1219676
CCGACGC657.3468604E-444.604168145
ACACGAG9000.044.329817
CACGAGC9000.043.523818
AAACACG10700.043.386785
TAACAAG29200.039.2496225
GCACGCA6100.039.240172
ACGAGCG750.001479638938.687839
AGTAACA30350.036.8063933
ACAAGAG33850.034.0732967
AAGAGCG6850.033.8871549
ACGAGCC2603.6379788E-1233.4798559
GTAGTTG16200.032.685461
AAGCGGT3350.032.4804577
Produced by FastQC (version 0.11.2)