FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_363_rr.352000000a97e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_363_rr.352000000a97e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411243
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4810.11696247717286373No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC100.007078192145.07646
ATCGGGC300.0019318272.529378
GCCGACC1200.066.43668145
GGATCGG350.003557573362.1756026
ACGAGCG350.003559291462.1680229
AAACACG3050.059.4575425
ACGAGCC1353.6379788E-1253.7254569
ACACGTG1004.6791683E-850.7705577
ACACGAG2750.050.11127
CACGAGC2800.049.216358
AACACGA3100.044.45896
GTCGCCC1652.7284841E-1143.925076145
AACACGT1201.9615072E-742.3139576
GAAACAC4300.042.1733744
CACGTGC1451.9163053E-840.01628
TAGAAAC7150.039.566292
ACGTGCC800.00203117736.2646839
TAACTAC1551.4576017E-632.75527
AAGCACG900.003618574532.2352757
TCTCGGG3850.032.002556145
Produced by FastQC (version 0.11.2)