FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_361_rr.352000000a97d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_361_rr.352000000a97d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781956
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT107690.6043359095286303No Hit
GTATGCACTCTGTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTC39650.22250829986823467No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG34390.1929901748415786No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC34280.1923728756490059No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT29450.16526782928422476No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT26410.14820792432585317No Hit
GTGCTTGTGATTCTAATTCACTTACATCATTGGCTCCCATTATGACCTCC21040.1180725001066244No Hit
ATGTTAATCCATCCCCTGTGGCCCACTTGGCATAATTGGTTTTCTGTAAT20870.11711849226355758No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT20820.11683790172147909No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG20700.11616448442049075No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG19950.11195562628931355No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT19540.10965478384427No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC19330.10847630356754039No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCAGCTGG19310.108364067350709No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA18860.10583875247200268No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC18560.10415520921953178No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCG151.226598E-4145.105749
AAACACG6800.088.557185
CGCACTT1050.082.917575
CACGCAC1150.082.016293
GCACGCA1250.075.450742
AGCACGC1250.075.450741
AACACGA7950.072.0965656
ACACGAG8200.069.8985067
GAAACAC9000.067.716014
CACGAGC8450.066.971888
ACGAGCC2500.060.9444129
ACGCACT1550.060.8507964
TGACGCC1251.8189894E-1257.98198145
GCCGACC553.224628E-452.71089145
TTATTCA33000.051.666449
GCGGAGC3350.049.8096161
CGGTGCA2100.048.3658642
ATTCGGG604.9537135E-448.318317145
GTGTCCT34700.048.08711
ACGAGCA9400.041.6793069
Produced by FastQC (version 0.11.2)