FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_348_rr.352000000a97c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_348_rr.352000000a97c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676303
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG18730.11173397649470293No Hit
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT18570.11077949511514326No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT18550.1106601849426983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT8350.095.561455
GTAGTAA30400.093.5298161
TAGTAAC31050.091.57462
GTAACAA36400.078.119814
AGCACGC10350.073.584531
TAACAAG38800.073.287665
ACGCACT12050.066.218934
AGTAACA43300.065.667243
AAGAGCG9850.063.3361559
GCACGCA12400.062.5911062
ACAAGAG57950.049.5714077
CAAGAGC58400.047.201988
AACACGA16450.044.0932041
AACAAGA66550.042.728196
CACGCAC18200.042.2459453
CTCGCTC6250.041.7804453
AAACACG15300.041.722255
TCGCTCT6550.040.9766964
GCGGTGC8800.039.563631
TCTCGCT6850.039.1797522
Produced by FastQC (version 0.11.2)