FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_347_rr.352000000a97bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_347_rr.352000000a97bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686405
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT27240.16152703532069698No Hit
ATACAGTGTCTGAGGAAGCCCAGATATTGGACACACTAGACAAATACTTT26200.1553600706829024No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT25210.14948959472961715No Hit
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG22740.13484305371485497No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA20930.1241101633356163No Hit
GAGTAACACCTGGGAAAGCTTCACCAGAGGGTCCTTTTCCACCACAGCAA20460.12132316970122836No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG18450.10940432458395226No Hit
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT18290.10845556079352231No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC33000.0110.3448642
GTAGTAA33550.0109.39751
CGCACTT14650.0101.511985
GTAACAA39150.094.131064
AGCACGC16150.092.970941
GCACGCA17200.086.876252
TAACAAG44150.083.6355
AAGAGCG10750.068.832379
CGAGCGG2450.065.141475
AGTAACA56700.064.737443
ACGCACT24700.061.0896154
ACGAGCG4350.056.700994
CGGTGCA7350.056.2535362
AACACGA24000.056.214861
CACGCAC27550.054.2417533
ACAAGAG68350.053.4941067
GCGGTGC11600.053.1508671
CGTGCGC701.6907137E-551.8155483
TCTCGCT7550.048.038052
GTAGAAA32950.045.7885931
Produced by FastQC (version 0.11.2)