FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_346_rr.352000000a97a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_346_rr.352000000a97a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126876
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT5800.057.5334135
GTAGTAA23200.051.9039231
TAGTAAC23100.050.87252
ACGAGCG1004.6860805E-850.778299
AGCACGC7450.046.737451
AACACGA8900.046.460666
ACGCACT7750.044.9293174
CGCCGTT1002.7410715E-643.487377145
CACGAGC10350.042.0524188
AAACACG12300.041.873915
GTAACAA28100.041.8214384
GCACGCA8950.040.525372
AAGAGCG9650.039.840859
GCCGACC1303.7094105E-739.027134145
ACACGAG11350.038.3473557
TAACAAG31000.038.143125
AGTAACA31650.036.6721653
ACGAGCC3350.030.3154019
TCGCCGG4200.029.336723145
GTAGAAA37850.028.7478521
Produced by FastQC (version 0.11.2)