FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_344_rr.352000000a9793.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_344_rr.352000000a9793.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560641
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT59440.3808691428714227No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC19220.12315452432686312No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT18650.1195021789123828No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT18130.11617021467461126No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT16830.10784030408018243No Hit
GATTCATTTCTGTTTGAACCCAAGCAGCCACTCTACATTTGTTAGGGATT15750.10092007066327233No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT2350.070.995665
AAACACG8450.064.3837665
AACACGA8100.062.688066
CGTATAG350.00355927962.176323
CGGTGCA1750.058.0312352
AGCGTTT1500.058.031235
ACGCACT3200.054.404284
AGCACGC2950.054.0969121
GTAACAA27650.053.2565044
GCACGCA3050.052.3232422
ACACGAG9850.050.812527
TAGTAAC29950.049.8932952
GTAGTAA31500.049.7410551
AGCCACC11950.049.132454145
TAACAAG30350.048.51875
TATACGG450.00961965848.359365
CACGAGC10450.047.8950548
GAAACAC12600.043.1784
ACAAGAG35450.042.151097
AGTAACA37400.040.3425673
Produced by FastQC (version 0.11.2)