FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_340_rr.352000000a9786.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_340_rr.352000000a9786.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412502
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC1300.061.33497145
CGCACTT3700.056.852255
AACACGA6250.051.065086
GCACGCA4550.051.0140652
AAACACG8150.045.3903925
TAGTAAC13700.045.0038572
ACACGAG7200.044.32897
CACGAGC7450.042.8413548
ACGCACT5250.042.830564
AGCACGC6250.039.457981
GTAGTAA16400.039.362451
GTAACAA17200.034.159224
CACGCAC7200.032.2380563
CGATATT1352.1454951E-532.216347145
ACGAGCC5250.031.7775129
GAAGCGG5300.031.4777246
AGAAGCG5450.030.6113645
AAGCGGT3950.029.3825637
GCGTTCA1000.00606072529.0142549
TAACAAG22000.027.6954215
Produced by FastQC (version 0.11.2)