FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_338_rr.352000000a9779.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_338_rr.352000000a9779.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931819
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA23070.11942112589222904No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA22840.11823053816118383No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG21240.10994818872782595No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG20850.10792936605344497No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT19750.10223525081801141No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCC2450.082.910869
AAACACG6650.079.6359945
ACACGAG5950.079.253027
AACACGA6450.073.107486
CACGAGC6650.070.91068
GAAACAC7500.070.610584
CACGTGC851.6916601E-1068.2795268
CGCACCC554.070538E-665.8886145
ACACGTG1007.166818E-1058.03767
GAGCAGA7050.057.6215061
AACACGT1051.1041266E-955.2724726
CACAGCG400.00603747754.4074333
TGACGCC1152.4938345E-950.4191145
TAGTAGA3050.047.569343
CGCCGTG1551.4551915E-1146.75965145
GCCGACC1109.9902536E-846.122025145
GAGCGTT657.314897E-444.6443067
AGAAGCG4850.043.3774955
CGGTGCA2150.040.4881972
GTAACAA24550.040.4834254
Produced by FastQC (version 0.11.2)