FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_337_rr.352000000a9736.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_337_rr.352000000a9736.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442615
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA43350.0100.059451
TAGTAAC43550.098.7671662
CGCACTT12250.095.333335
GTAACAA50200.085.396244
TAACAAG58900.072.7825245
AGTAACA59850.071.8696443
ACGCACT17750.066.610734
GCACGCA17150.065.133232
AGCACGC17200.064.1004641
AAGAGCG13100.060.355819
AAACACG23300.051.9894875
ACAAGAG86650.049.642087
CAAGAGC87150.048.275248
CACGCAC23300.047.9423983
AACAAGA97000.044.493876
GACGAAA4600.042.54507145
AACACGA24950.042.446056
GTAGAAA45000.041.2642061
CGAGCTA3350.038.9746865
CGGTGCA7950.038.3202362
Produced by FastQC (version 0.11.2)