FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_333_rr.352000000a9729.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_333_rr.352000000a9729.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979722
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC56570.2857471907671885No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA45270.22866846961341036No Hit
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT43560.2200308932264227No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA35240.1780047905716055No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT33020.16679109491130573No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATCCTTTTAC31150.15734532424249464No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC31070.15694122710158295No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG29490.1489603085685768No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT29060.1467882864361764No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA28040.14163604788955217No Hit
GTATTCAGGGTCCATTAGATTGTACTTCAACACTATTTCTGGGATATGCA26830.13552407863326266No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT26500.13385717792700189No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC24260.12254245798147417No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT23990.12117863013089718No Hit
CCCCATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG23080.11658202515302653No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA22640.11435949087801216No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT21930.1107731287524208No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT21530.10875264304786227No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA21440.10829803376433661No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT21410.10814649733649472No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA21160.10688369377114565No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT20050.10127684594099576No Hit
CCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT20040.1012263337983818No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG10850.083.58395
CGGTGCA3950.080.814062
AACACGA11700.073.791016
GAAACAC12500.073.1312264
ACACGAG13350.064.670777
AGCGTGT451.19001226E-464.489628
TCGCGCC350.003571776262.121956145
CACGAGC14550.058.8384678
GTAACAA28300.058.1944774
GGTGCAT5350.056.9543463
GCGGTGC9200.054.411741
GCGGAGC5650.053.9302141
AGAAGCG5600.053.117565
ATTCGGG701.6999746E-551.768295145
TGACGCC1152.4938345E-950.41782145
ACGAGCC7900.049.59179
GGCGCGA450.0096148348.3659861
TAGTAAC36500.046.5108572
ACAAGAG36050.046.0863727
ACGAGCA10850.043.4636279
Produced by FastQC (version 0.11.2)