FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_328_rr.352000000a970f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_328_rr.352000000a970f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092431
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT90940.43461409241212734No Hit
GCTTTGGTCATGTCATATCCTTGTATCGATGATTCTTGTTCAACAATTGC32100.15341007660467657No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC31970.1527887896900782No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG31760.1517851723664962No Hit
GTATGCACTCTGTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTC28090.13424576485437273No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT26160.12502204373764295No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT25400.1213899048522986No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATCCTTTTAC24870.11885696589278213No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG24850.11876138329053622No Hit
GGGGCTATTTACTCTGTCTCCCTTGAAACAAGCTTTGGTCATGTCATATC24410.11665856604112634No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT23010.10996778388391301No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA22910.1094898708726835No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT22510.1075782188277654No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG7200.057.4335635
GCCGACC1051.1132215E-955.224163145
CGCACTT1450.055.0361565
TGACGCC1206.002665E-1154.361282145
CGGTGCA3700.050.976992
AACACGA7900.050.507866
GCACGCA1650.048.3627932
AGCACGC1650.048.3593141
ACACGAG8300.048.073757
TTATTCA28550.046.754649
CACGAGC8900.044.832828
GAAACAC9500.043.5285954
ACGATCC1703.8198777E-1142.6363145
CACGCAC1953.6379788E-1240.9243163
GCGGAGC4150.040.2023241
AAGCGGT4250.039.2610867
GTAACAA30850.037.861174
AGAAGCG5450.037.272195
GTGTCCT35100.036.7861481
GCGGTGC6000.036.2694851
Produced by FastQC (version 0.11.2)