FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n02_s1_327_rr.352000000a96e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n02_s1_327_rr.352000000a96e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288558
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT60360.2637468659304243No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT36330.15874624982194027No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT29300.12802821689465593No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT27360.1195512632845661No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA26830.11723539451479928No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG26450.11557496030251363No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA25850.11295322207258893No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT25740.11247257006376941No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC25180.11002561438250635No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA24920.10888952781620566No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC24630.10762235433840873No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC24620.10757865870124332No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT24210.10578713757746144No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCC100.0070946324144.97827145
TGTCGTG100.0070946324144.97827145
GAGCAGA4600.099.352491
AAACACG9650.091.726585
GAAACAC9700.091.253754
CGGTGCA3750.090.924652
CAGGATT2200.089.043815
GGTGCAT3850.088.564923
ACGCACT259.3604653E-487.065064
GTAACAA17950.084.073964
ACACGTG554.3748514E-879.148327
AACACGA10950.076.861096
ACACGAG11350.074.1506967
GCTCGGG300.001937212772.48914145
CGTGCCC502.3092452E-672.489136145
GGATCCC702.8176146E-972.489136145
TAGAAAC13400.070.380712
CACGAGC12800.065.750828
CACAGGA3150.062.1838843
CACGTGC1600.058.941261
Produced by FastQC (version 0.11.2)